新甾体皂苷的提取及其细胞毒性研究

2018年3月，第四军医大学药学院药物研究所；氟氮化工国家重点实验室；西安现代化学研究所 (Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, Xi’an 710032, China；State Key Laboratory of Fluorine & Nitrogen Chemicals, Xi’an 710065, China；Xi’an Modern Chemistry Research Institute, Xi’an 710065, China) Haifeng Tang老师研究团队在《Molecules》上发表论文：

“New Steroidal Saponins from the Rhizomes of Paris vietnamensis and Their Cytotoxicity”

“新甾体皂苷的提取及其细胞毒性研究”

Abstract：

Four new spirostanol saponins, named pavitnosides A-D (1-4), with six known steroidal saponins 5-10 were isolated from the rhizomes of Paris vietnamensis. Their chemical structures were determined based on extensive spectroscopic studies and chemical methods. The aglycones of pavitnoside B and pavitnoside C were not reported in previous work. The cytotoxicity of all saponins was evaluated against human glioblastoma U87MG and U251 cell lines. The new spirostanol saponin 1 displayed weak anti-proliferative activity against U87MG cell line and the known saponins 8 and 9 exhibited significant cytotoxicity against the two tumor cell lines, with IC50 values of 2.16 to 3.14 μM, but did not affect the growth of primary cultures of human astrocytes.

摘要：

从越南梧桐根茎中分离到4个新的螺甾醇类皂苷，命名为pavitnosides A-D(1-4)， 6个已知甾体类皂苷5-10。它们的化学结构是根据广泛的光谱研究和化学方法确定的。前文未报道过其苷元B和C的苷元。评价了各皂苷对人胶质母细胞瘤U87MG和U251细胞系的细胞毒性。新合成的螺甾醇皂苷1对U87MG细胞系具有较弱的抗增殖活性，而已知的皂苷8和皂苷9对两种肿瘤细胞系具有明显的细胞毒性，IC50值为2.16 ~ 3.14 μM，但对人星形胶质细胞原代培养物的生长没有影响。

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