一种灭活的新型基因型禽腺病毒4对最近在中国出现的鸡心包积液综合征具有保护作用

2017年8月，中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室；江苏省重大动物传染病与人畜共患病预防控制协同创新中心(State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China;   Jiangsu Co Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou 225009, China) Xiaomei Wang老师研究团队在《Viruses》上发表论文：

“An Inactivated Novel Genotype Fowl Adenovirus 4 Protects Chickens against the Hydropericardium Syndrome That Recently Emerged in China”

“一种灭活的新型基因型禽腺病毒4对最近在中国出现的鸡心包积液综合征具有保护作用”

Abstract：

Since 2015, China has experienced outbreaks of severe hydropericardium syndrome (HPS), associated with a novel genotype and hypervirulent fowl adenovirus serotype 4 (FAdV-4) infection, with a prevalence in various provinces of the country. This has resulted in huge economic losses in the poultry industry. The novel FAdV-4 showed new genome characters, such as the natural deletion of open reading frame (ORF) 19 and ORF 27 (1966 bp), and high pathogenicity toward chickens. These are coupled with severe hydropericardium, inclusion body hepatitis, and mortality rates ranging from 30% to 90%. Although several inactivated and subunit vaccines against the traditional FAdV-4 have been developed, no commercial vaccine against the emerged disease caused by the novel strain has been available until now. The potential risks of infection with this novel hypervirulent FAdV-4 urgently require an effective vaccine. Thus, an inactivated oil-emulsion FAdV-4 vaccine formulated with the novel genotype virus was developed in this study. The vaccine provided a high level of antibody, preferential T helper 2 (Th2) (interleukin-4 secretion) not Th1 (interferon-γ secretion) response, and full protection against a lethal dose of the novel hypervirulent FAdV-4. Therefore, the novel genotype FAdV-4 vaccine is proposed as an attractive candidate to prevent and reduce the spread of HPS in the poultry industry of China.

摘要：

自2015年以来，中国爆发了严重心包积液综合征(HPS)，与一种新型基因型和高毒力禽腺病毒血清型4 (FAdV-4)感染有关，在全国多个省份流行。这给家禽业造成了巨大的经济损失。新型FAdV-4具有自然缺失开放阅读框(ORF) 19和ORF 27 (1966 bp)等新的基因组特征，对鸡具有较高的致病性。伴有严重心包积液、包涵体肝炎，死亡率从30%到90%不等。虽然已经开发出几种针对传统FAdV-4的灭活疫苗和亚单位疫苗，但迄今为止还没有针对这种新菌株引起的新出现疾病的商业疫苗。这种新型高毒力FAdV-4感染的潜在风险迫切需要一种有效的疫苗。因此，本研究研制了以新基因型病毒配制的FAdV-4灭活油乳剂疫苗。该疫苗提供了高水平的抗体，优先的辅助性T细胞2 (Th2)(白细胞介素-4分泌)而不是Th1(干扰素-γ分泌)反应，并对致命剂量的新型高致病性FAdV-4具有充分的保护作用。因此，新型FAdV-4基因型疫苗被认为是预防和减少HPS在中国家禽业传播的有吸引力的候选疫苗。

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